Usefulness of dark field microscopy (DFM), IGM ELISA and microscopic agglutination test (MAT) for the early diagnosis of acute leptospirosis

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Assessment of the efficacy of an IgM-elisa and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis.

In a prospective study in Barbados between 1979 and 1989, 321 cases were diagnosed in 638 patients presenting at a hospital with symptoms of leptospirosis. Initial diagnosis was based on patient history and characteristic signs and symptoms. In 92 cases (29%), diagnosis was confirmed by isolation of organisms from the blood, urine, or dialysate fluid; in the remaining 229 cases (71%) diagnosis ...

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Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test

BACKGROUND Leptospirosis is diagnosed on clinical grounds, and confirmed by microscopic agglutination test (MAT). IgM-ELISA (Serion-Virion) and immunochromatography test (Leptocheck-WB) are two immunodiagnostic assays for leptospirosis. Their sensitivity, specificity and applicability in Sri Lanka have not been systematically evaluated. METHODS Clinically diagnosed leptospirosis patients (n =...

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Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis

Background: Leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit with an in-house ELISA assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms. Methods: A total of 282 serum samples of patients suspicious of leptospirosis admitt...

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Serologic and bacteriologic diagnosis of bovine leptospirosis in Tehran suburb dairy farms

Leptospirosis is a worldwide zoonosis caused by Leptospira interrogans. This study was conducted toevaluate serologic and bacteriologic findings of leptospirosis in clinically-suspected cows. 380 sera and 33urine samples were collected from 6 industrial dairy farms in Tehran suburb, from December 2004 to June2005. The prevalence of disease was determined by microscopic agglutination test (MAT),...

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ژورنال

عنوان ژورنال: Indian Journal of Microbiology Research

سال: 2019

ISSN: 2394-546X,2394-5478

DOI: 10.18231/j.ijmr.2019.036